Ecosystem screening approach for pathogen-associated microorganisms affecting host disease.
Identifieur interne : 001760 ( Main/Exploration ); précédent : 001759; suivant : 001761Ecosystem screening approach for pathogen-associated microorganisms affecting host disease.
Auteurs : Eric Galiana [France] ; Antoine Marais ; Catherine Mura ; Benoît Industri ; Gilles Arbiol ; Michel PonchetSource :
- Applied and environmental microbiology [ 1098-5336 ] ; 2011.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Ascomycota (croissance et développement), Biofilms (croissance et développement), Données de séquences moléculaires (MeSH), Interactions microbiennes (MeSH), Maladies des plantes (microbiologie), Microbiologie du sol (MeSH), Oligohymenophorea (croissance et développement), Phytophthora (croissance et développement), Phytophthora (pathogénicité), Racines de plante (microbiologie), Rhizosphère (MeSH), Tabac (microbiologie), Écosystème (MeSH).
- MESH :
- croissance et développement : Ascomycota, Biofilms, Oligohymenophorea, Phytophthora.
- microbiologie : Maladies des plantes, Racines de plante, Tabac.
- pathogénicité : Phytophthora.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Interactions microbiennes, Microbiologie du sol, Rhizosphère, Écosystème.
English descriptors
- KwdEn :
- Ascomycota (growth & development), Biofilms (growth & development), Ecosystem (MeSH), Microbial Interactions (MeSH), Molecular Sequence Data (MeSH), Oligohymenophorea (growth & development), Phytophthora (growth & development), Phytophthora (pathogenicity), Plant Diseases (microbiology), Plant Roots (microbiology), Rhizosphere (MeSH), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH), Tobacco (microbiology).
- MESH :
- growth & development : Ascomycota, Biofilms, Oligohymenophorea, Phytophthora.
- microbiology : Plant Diseases, Plant Roots, Tobacco.
- pathogenicity : Phytophthora.
- Ecosystem, Microbial Interactions, Molecular Sequence Data, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology.
Abstract
The microbial community in which a pathogen evolves is fundamental to disease outcome. Species interacting with a pathogen on the host surface shape the distribution, density, and genetic diversity of the inoculum, but the role of these species is rarely determined. The screening method developed here can be used to characterize pathogen-associated species affecting disease. This strategy involves three steps: (i) constitution of the microbial community, using the pathogen as a trap; (ii) community selection, using extracts from the pathogen as the sole nutrient source; and (iii) molecular identification and the screening of isolates focusing on their effects on the growth of the pathogen in vitro and host disease. This approach was applied to a soilborne plant pathogen, Phytophthora parasitica, structured in a biofilm, for screening the microbial community from the rhizosphere of Nicotiana tabacum (the host). Two of the characterized eukaryotes interfered with the oomycete cycle and may affect the host disease. A Vorticella species acted through a mutualistic interaction with P. parasitica, disseminating pathogenic material by leaving the biofilm. A Phoma species established an amensal interaction with P. parasitica, strongly suppressing disease by inhibiting P. parasitica germination. This screening method is appropriate for all nonobligate pathogens. It allows the definition of microbial species as promoters or suppressors of a disease for a given biotope. It should also help to identify important microbial relationships for ecology and evolution of pathogens.
DOI: 10.1128/AEM.05371-11
PubMed: 21742919
PubMed Central: PMC3165409
Affiliations:
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microbiennes (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Microbiologie du sol (MeSH)</term><term>Oligohymenophorea (croissance et développement)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (pathogénicité)</term><term>Racines de plante (microbiologie)</term><term>Rhizosphère (MeSH)</term><term>Tabac (microbiologie)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Ascomycota</term><term>Biofilms</term><term>Oligohymenophorea</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ascomycota</term><term>Biofilms</term><term>Oligohymenophorea</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Racines de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Roots</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ecosystem</term><term>Microbial Interactions</term><term>Molecular Sequence Data</term><term>Rhizosphere</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Interactions microbiennes</term><term>Microbiologie du sol</term><term>Rhizosphère</term><term>Écosystème</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microbial community in which a pathogen evolves is fundamental to disease outcome. Species interacting with a pathogen on the host surface shape the distribution, density, and genetic diversity of the inoculum, but the role of these species is rarely determined. The screening method developed here can be used to characterize pathogen-associated species affecting disease. This strategy involves three steps: (i) constitution of the microbial community, using the pathogen as a trap; (ii) community selection, using extracts from the pathogen as the sole nutrient source; and (iii) molecular identification and the screening of isolates focusing on their effects on the growth of the pathogen in vitro and host disease. This approach was applied to a soilborne plant pathogen, Phytophthora parasitica, structured in a biofilm, for screening the microbial community from the rhizosphere of Nicotiana tabacum (the host). Two of the characterized eukaryotes interfered with the oomycete cycle and may affect the host disease. A Vorticella species acted through a mutualistic interaction with P. parasitica, disseminating pathogenic material by leaving the biofilm. A Phoma species established an amensal interaction with P. parasitica, strongly suppressing disease by inhibiting P. parasitica germination. This screening method is appropriate for all nonobligate pathogens. It allows the definition of microbial species as promoters or suppressors of a disease for a given biotope. It should also help to identify important microbial relationships for ecology and evolution of pathogens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21742919</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5336</ISSN><JournalIssue CitedMedium="Internet"><Volume>77</Volume><Issue>17</Issue><PubDate><Year>2011</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Ecosystem screening approach for pathogen-associated microorganisms affecting host disease.</ArticleTitle><Pagination><MedlinePgn>6069-75</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/AEM.05371-11</ELocationID><Abstract><AbstractText>The microbial community in which a pathogen evolves is fundamental to disease outcome. Species interacting with a pathogen on the host surface shape the distribution, density, and genetic diversity of the inoculum, but the role of these species is rarely determined. The screening method developed here can be used to characterize pathogen-associated species affecting disease. This strategy involves three steps: (i) constitution of the microbial community, using the pathogen as a trap; (ii) community selection, using extracts from the pathogen as the sole nutrient source; and (iii) molecular identification and the screening of isolates focusing on their effects on the growth of the pathogen in vitro and host disease. This approach was applied to a soilborne plant pathogen, Phytophthora parasitica, structured in a biofilm, for screening the microbial community from the rhizosphere of Nicotiana tabacum (the host). Two of the characterized eukaryotes interfered with the oomycete cycle and may affect the host disease. A Vorticella species acted through a mutualistic interaction with P. parasitica, disseminating pathogenic material by leaving the biofilm. A Phoma species established an amensal interaction with P. parasitica, strongly suppressing disease by inhibiting P. parasitica germination. This screening method is appropriate for all nonobligate pathogens. It allows the definition of microbial species as promoters or suppressors of a disease for a given biotope. It should also help to identify important microbial relationships for ecology and evolution of pathogens.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Galiana</LastName><ForeName>Eric</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1301 Interactions Biotiques et Santé Végétale, F-06903 Sophia Antipolis, France. galiana@sophia.inra.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marais</LastName><ForeName>Antoine</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Mura</LastName><ForeName>Catherine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Industri</LastName><ForeName>Benoît</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Arbiol</LastName><ForeName>Gilles</ForeName><Initials>G</Initials></Author><Author 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uniqKey="Ponchet M" first="Michel" last="Ponchet">Michel Ponchet</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Galiana, Eric" sort="Galiana, Eric" uniqKey="Galiana E" first="Eric" last="Galiana">Eric Galiana</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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